Detection of fastidious infectious enteric viruses in water
Article Abstract:
A technique utilizing the continuous cell line of CaCo-2, derived from a human colon adenocarcinoma, combined with molecular hybridization with specific DNA probes was developed to detect fastidious enteric viruses in freshwater samples. The CaCo-2 cells, which have increased sensitivity to virus laboratory strains, detected wild-type rotaviruses, enteric adenoviruses, enteroviruses and astroviruses in the samples. This indicates that utilization of CaCo-2 cells is an effective in vivo method for detecting and monitoring infectious viruses in the environment.
Publication Name: Environmental Science & Technology
Subject: Science and technology
ISSN: 0013-936X
Year: 1995
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Identifying the major sources of nutrient water pollution
Article Abstract:
A 1992 evaluation showed that around 44% of US river miles did not conform with environmental standards set by states, despite the use of over $540 billion since 1972 to establish water pollution controls. Non-compliance with the Clean Water Act provisions has been related to inadequate controls for nonpoint-source pollution. Evaluation of nitrogen and phosphorus source points established the need for specific watershed management programs to deter pollution.
Publication Name: Environmental Science & Technology
Subject: Science and technology
ISSN: 0013-936X
Year: 1995
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