Rotational motions of macromolecules by single-molecule fluorescence microscopy
Article Abstract:
A brief review of methods to detect angles and rotational motions of single fluorophores is presented and give an example of three-dimensional, total internal reflection, single-molecule fluorescence polarization applied to actin as it is translocated by conventional muscle myosin. Several complementary techniques are developed to determine average orientation dynamics on multiple time scales, and concerted rotational motions of individual fluorescent probes bound to biological macromolecules.
Publication Name: Accounts of Chemical Research
Subject: Science and technology
ISSN: 0001-4842
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Molecular recognition imaging and force spectroscopy of single biomolecules
Article Abstract:
The capability of atomic force microscope (AFM) to resolve nanometer-sized details, together with its force detection sensitivity, has developed molecular recognition imaging and by a combination of topographical imaging with force measurements receptor sites are localized with nanometer accuracy. Topography and recognition of target molecules are thereby simultaneously mapped as AFM can identify specific components in a complex biological sample and retain its high resolution in imaging.
Publication Name: Accounts of Chemical Research
Subject: Science and technology
ISSN: 0001-4842
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: International technology transfer in small business: a new paradigm. Technology transfer to hotels in China by multinational hotel enterprises in Hong Kong
- Abstracts: Evaluation of the mechanical properties and corrosion resistance of Al-2.5 Mg-Cr alloys. Corrosion fatigue of AZ91E-T6 cast magnesium alloy in a 3.5 percent NaCl aqueous environment
- Abstracts: Supramolecular chemistry. Molecular trees: a new branch of chemistry. High-resolution imaging of the self-assembly of organic monolayers
- Abstracts: Formation of hydrocarbons from the reduction of aqueous CO2 by zero-valent iron. Chemical mixtures of fuel-rich and fuel-lean flames of CHCl3/CH4
- Abstracts: Surfact-enhanced Raman scattering in local optical fields of silver and gold nanoaggregates - form single-molecule Raman spectroscopy to ultrasensitive probing in live cells