Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner

Article Abstract:

Various analyses are conducted to study the transcription dynamics of single RNA polymerase II (RNAP II) molecules against force and in the presence and absence of a transcription elongation factor that increases transcription through nucleosomal barriers. The results show that the enzyme backtracking plays a very important role in response of RNAP II to a hindering force, as its mechanical performance is highly affected by the transcription factors.

Analysis, RNA polymerases, Molecular dynamics, Force and energy, Speed, Forces (Physics)

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DNA overwinds when stretched

Article Abstract:

A study shows that for small distortions DNA overwinds under tension, reaching a maximum twist at a tension of approximately 30pN. The results have implications for the action of DNA-binding proteins that must stretch and twist DNA to compensate for variability in the lengths of their binding sites and the requisite coupled DNA distortions are favored by the intrinsic mechanical properties of the double helix.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, Usage, DNA, Fluorescence microscopy, Mechanical properties, Structure

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Mechanochemical analysis of DNA gyrase using rotor bead tracking

Article Abstract:

The activity of gyrase in real time is observed by tracking the rotation of a submicrometre bead attached to the side of a stretched DNA molecule. In the presence of gyrase and ATP, bursts of rotation is observed corresponding to the processive, stepwise introduction of negative supercoils in strict multiples of two.

Molecular structure, Adenosine triphosphate, ATP, DNA-ligand interactions, DNA binding

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