Conversion of diploidy to haploidy
Article Abstract:
Converting the human chromosome complement to a haploid state through fusion to a novel recipient cell line makes it possible to overcome the problem of mutations in one copy of chromosome pair being obscured by the normal sequence present on the other copy of the chromosome. The conversion approach uses fusion between human and rodent cells to form hybrids that have only one subset of the human chromosomes. It has been possible to devise a reliable system involving a new recipient cell line for producing stable hybrids with any desired chromosome after a single fusion with a universal recipient. Diploid-to-haploid conversion can be used to maximize the sensitivity of existing methods of searching for mutations.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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CpG methylation is maintained in human cancer cells lacking DNMT1
Article Abstract:
The mechanism for hypermethylation in several forms of cancer is poorly understood, but the prototypic DNA methyltransferase DNMT1 is thought to be responsible. Research is presented that tests this hypothesis.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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Mutations in a signaling pathway
Article Abstract:
The analysis of three hundred and forty genes encoding serine/threonine kinases (STKs) from human genome for mutations in tumors from colorectal cancer patients is discussed. Gene mutations affected three altered genes namely, phosphoinositide-dependent protein kinase-1 (PDK 1), v-akt murine thymoma viral oncogene homologue-2 kinase (AKT 2) and p21-activated kinase 4 (PAK 4), involved in the phosphatidylinositol-3-OH kinase (PI(3)K) signaling pathway.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2005
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