Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase
Article Abstract:
The substrate 1,2 dichloroethane has been used to determine the crystal structure of haloalkane dehalogenase. The importance of haloalkane dehalogenase in the degradation of halogenated materials such as those produced by cleaning compounds, solvents and pesticides. Studies have shown that the substrate is linked to the active site without being converted at ph 5 and a temperature of 4 degrees centigrade. However, when pH is increased to 6 and temperature brought to room temperature levels, the carbon-chlorine bond is broken to produce the residue Asp124. The results of these studies show that dehalogenase catalization is caused by a tw-step mechanism. (how enlightening)
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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Crystallographic structure of the T domain-DNA complex of the Brachyury transcription factor
Article Abstract:
The x-ray structure of the T domain from Xenopus laevis is reported, in complex with the 24-nucleotide palindromic DNA duplex. The protein is bound as dimer and interacts with major and minor DNA grooves. the carboxy-terminal helix is embedded deeply into the enlarged minor groove without DNA bending, indicating a new type of specific DNA contact, and revealing a new way that a protein can recognize DNA.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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Crystallographic antibodies
Article Abstract:
New types of crystals produced by antibodies simulated by different unrelated crystals have been discovered. This is an essential process that is involved in the specific reaction of proteins with the surface or nuclei of molecule crystals. This is a fundamental process of growth and development in all living animals.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1995
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