Movement of 'gating charge' is coupled to ligand binding in a G-protein-coupled receptor

Article Abstract:

Heterologous expression in Xenopus oocytes is used to show that two prototypical G-protein coupled receptors (GPCRs), the m2 and m1 muscarinin receptors (m2R and m1R), display charge movement associated currents analogous to gating currents of voltage gated channels. The loop that couples m2R and m1R to their G protein has a crucial function in coupling voltage sensing to agonist-binding affinity indicating that GPCRs serve as sensors for both transmembrane potential and external signals.

Genetic aspects, Electric properties, Protein binding, G proteins, Oocytes, GABA, GABA agonists

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From molecule to malady

Article Abstract:

Ion channels are membrane proteins that are of crucial physiological importance. An explosion in the number of crystal structures on ion channels has led to a marked increase in understanding how ion channels open and close and select between permeant ions, and research on channelopathies or diseases resulting from impaired channel function identifies mutations in over 60 ion-channel genes that cause human disease.

Analysis, Crystals, Crystal structure

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Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement

Article Abstract:

Fluorescence resonance energy transfer (FRET) is used between the fourth transmembrane helix (S4) domain and a reference point in the lipid bilayer for determining the extent of transmembrane movement of S4. The non-translocation of S4 segment across the lipid bilayer is indicated as no transient fluorescence is observed in Shaker K(super +) channels.

United States, Lipid membranes, Energy transformation

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