Pest control by fluorescence
Article Abstract:
The green fluorescent protein (GFP) introduced into the Autographa californica multiple nuclear polyhedrosis virus, is useful for the biological control of lepidopterous pests. The baculovirus causes larval death and the green fluorescence produced by GFP on excitation with light is used for the early detection of infected individuals from healthy larvae. GFP eliminates the need of molecular analysis to detect the presence of viruses in the larvae. Baculovirus containing similar GFPs can be used to predict the dispersal and presence of virus that adversely affects the environment.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
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Simultaneous independent measurement of endocytosis and exocytosis
Article Abstract:
The simultaneous independent measurement of endocytosis and exocytosis of bovine adrenal chromaffin cell secretory granules shows that exocytosis ceases before endocytosis begins when the stimuli is small. FM1-43 fluorescence and capacitive monitoring are used to determine unidirectional movements of the membrane in bovine adrenal chromaffin cells. The linear increase in fluorescence and capacitance reveals that the start of endocytosis is delayed by 2-3 minutes during prolonged stimulation. The rate of endocytosis quickly equals that of exocytosis after the delayed onset.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
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Regulation of receptor-mediated endocytosis by Rho and Rac
Article Abstract:
The Ras-related G proteins, Rho and Rac, suppress the endocytosis regulated by transferrin receptors in intact and perforated cells. The accumulation of Texas red-conjugated transferrin decreases in cells expressing the activated GTP-forms of Rho and Rac but is unaffected in cells expressing wild-type or inactive proteins. The proteins suppress the rate and level of budding of clathrin-coated vesicles that are involved in endocytosis. The inhibitory activity of Rac is independent of Rho activity. A downstream effector regulates the effects of Rho and Rac.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
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