Rapid communication: PCR-RFLP for MspI and NcoI in the ovine calpastatin gene

Article Abstract:

Nucleotide sequencing of the PCR amplimers from the blood of six Dorset Down sheep was conducted. Exon and intron areas from part of the first repetitive domain of the ovine calpastatin gene were amplified to a product of 622 bp. Two alleles were detected that differ by having a number of restriction sites present in one allele but absent in the other. Given the role of calpastatin in bovine meat, the polymorphisms reported in the study might be used for marker-assisted sheep selection.

Hemoglobins, Sheep, Animal genetics, Hemoglobin polymorphisms

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Rapid communication: three unique restriction fragment length polymorphisms of EcoRI, PvuII, and ScaI digested mitochondrial DNA of bactrian camels (Camelus bactrianus ferus) in China

Article Abstract:

Research was conducted to examine the blood collected from two wild Bactrian camels and nine domestic Bactrian camels. The purification of mtDNA through digestion with SacI was followed by electrophoresis in 1% low-melting-point agarose gel. Results suggest that wild camels still exist in China despite the fact that the genotypes of the two animals, which include body structure, color and conformation, share many similarities.

Mitochondrial DNA, Camels

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Rapid communication: a TaqI restriction fragment length polymorphism in the bovine calpastatin gene

Article Abstract:

A cDNA probe was cloned by reverse transcriptase-polymerase chain reaction from bovine skeletal muscle and used as a bovine calpastatin probe. Four polymorphism bands of sizes 14.0, 9.0, 8.5, and 5.0 kb were found, while alleles of size 14.0, 9.0 + 5.0, and 8.5 + 5.0 KB bands were also discovered. The calpastatin/TaqI combination type was assigned to 385 unrelated cattle of six breeds and two breed crosses.

Cattle

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