Visualizing spatially correlated dynamics that directs RNA conformational transitions
Article Abstract:
The execution of the scheme, using nuclear magnetic resonance (NMR), to observe the dynamics between two RNA helices that are connected by a functionally significant trinucleotide bulge over timescales extending up to milliseconds is discussed. The 3D dynamic pathway is planned at different positions by seven separate ligand-bound conformations of the RNA and so even partly unstructured RNAs can experience structural dynamics that directs ligand-induced changes along specific predefined conformational pathways.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2007
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Relating ligand binding to activation gating in CNGA2 channels
Article Abstract:
The fluorescent cGMP analogue 8-DY547-cGMP as the ligand was used to study the action of homotetrameric olfactory-type cyclic-nucleotide gated (CNG)A2 channels in inside-out membrane patches by simultaneously determining channel activation and ligand binding. Steady-state binding was larger than steady-state activation at concentrations of 8-DY547-cGMP<1 [mu]M whereas at higher concentrations it was smaller, generating a crossover of the steady-state relationships.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2007
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Open-to-closed transition in apo maltose-binding protein observed by paramagnetic NMR
Article Abstract:
The domain rearrangement in the crystal structure of apo and holo maltose-binding protein is illustrated.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2007
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