Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease
Article Abstract:
Multiple gene products are essential in damage-specific recognition and DNA incision when yeast and mammalian cells undergo nucleotide excision repair. At least 6 genes are required for nucleotide excision repair in the yeast Saccharomyces cerevisiae, including Rad1 and Rad10. When both genes are mutated, nucleotide excision repair is inactivated and the efficiency of mitotic recombination between repeated sequences is reduced. The ability of purified Rad1 and Rad10 proteins to degrade single-stranded DNA by an endonucleolytic mechanis was demonstrated.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination
Article Abstract:
It has been established that XRCC2, a mammalian gene implicated in repair DNA double-strand break repair by homologous recombination on the basis of its sequence homology to yeast Rad51, is vital for the efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids. It is suggested that XRCC2 and other Rad51-related proteins could promote, but are not vital for, Rad51 activity. This may also be the case with other recombination proteins.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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Frequent chromosomal translocations induced by DNA double-stranded breaks
Article Abstract:
A mouse embryonic stem cell system has been used to investigate the role of DNA damage in the formation of translocations. Interchromosomal DNA repair does not lead to genome instability in itself, but the presence of two double-strand breaks in a single cell can change the spectrum of repair products that are recovered.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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