Discrimination of Listeria monocytogenes from other Listeria species by ligase chain reaction
Article Abstract:
Two pairs of primers were designed for use in a ligase chain reaction (LCR) assay to discriminate Listeria monocytogenes from other Listeria species. The primers were based on the 16s rRNA gene containing a single base-pair difference in theV9 region. Prior to LCR, the probes were amplified using the polymerase chain reaction. Tests on 19 different Listeria species showed that the procedure could effectively discriminate between species, and can therefore be a useful diagnostic tool for detecting Listeria monocytogenes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples
Article Abstract:
A PCR procedure based on 16S rRNA gene sequencing was used to detect 12 anaerobic bacteria in human and animal feces. The technique, which is simple and quick, is specific for these anaerobic bacteria that occur in human intestinal tracts. The results of the procedure compare well with those obtained from the usual more tedious procedures for the detection of the bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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