A UV-induced mutation in neurospora that affects translational regulation in response to arginine

Article Abstract:

A study of ultraviolet-induced mutants of the Neurospora crass arg-2 gene, developed in the presence of hygromycin and arginine, was conducted to determine the mechanics of translational regulation. One mutant which was similar to the arg-2-hph fusion gene responsible for encoding hygromycin phosphotransferase was recovered using polymerase chain reaction. Analyses of the mutant showed that it occurred at an upstream open reading frame (uORF) codon 12 in the 5' region and resulted in an abrogation of negative regulation. It suggests that loss of regulation featured a translational component and that the uORF sequence was essential in arginine-specific translation regulation.

Arginine, Genetic translation, Translation (Genetics)

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Spontaneous mutation during the sexual cycle of Neurospora crassa

Article Abstract:

Examination of spontaneous mutation during the sexual cycle of Neurospora crassa suggests the presence of three classes of sexual-cycle-specific mutations, which account for 50% of the mutation in the sexual-cycle mutational spectrum. Sequential analysis helps determine the DNA sequences of 42 spontaneous mutations of the methyltryptophan resistance gene, the structural gene for the neutral amino acid permease in Neurospora. Sexual-cycle mutational spectrum of Neurospora is compared with the spectrum of vegetative mutants.

Microbial mutation, Sexual cycle

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Cloning and analysis of the alternative oxidase gene of Neurospora crassa

Article Abstract:

Genomic and cDNA copies of the cyanide-resistant alternative respiratory pathway contained within the mitochondria of Neurospora crassa were isolated and analyzed. The alternative oxidase genes grown in the presence of an inhibitor of mitochondrial protein synthesis, chloramphenicol, had more mRNA than those grown in wild-type cultures. Sequence analyses of the mutant alleles suggest that the amount of mRNA is dependent on the presence of chloramphenicol.

Cloning

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