A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(sup)70
Article Abstract:
Results indicate that the beta' coiled-coil and sigma(sup)70 region interact and promote an allosteric transition that enables sigma(sup)70 to selectively recognize the nontemplate strand. Data reveal that a 48 amino acid coiled-coil of the beta' subunit induces sigma(sup)70 to perform the task as efficiently as core RNA polymerase.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2001
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Luminescence resonance energy transfer-based high-throughput screening assay for inhibitors of essential protein-protein interactions in bacterial RNA polymerase
Article Abstract:
This article describes a homogeneous assay for sigma factor binding to RNA polymerase based on luminescence resonance energy transfer. Data indicate that the assay can be performed in a multiwell plate and read by amultiplate reader enabling it to be a robust high-throughput screening assay for protein-proein interaction.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain
Article Abstract:
Results show that outer-membrane porins-like C-terminal sequences bind to DegS protease PDZ domain and activate DegS cleavage of transmembrane protein RseA to induce sigma(sup)E -dependent transcription. Data indicate that DegS senses envelope stress by binding to unassembled outer membrane porins.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2003
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