Mcm10 and the MCM1-7 complex interact to initiate DNA synthesis and to release replication factors from origins

Article Abstract:

A replication initiation protein that physically interacts with members of the MCM2-7 complex, a complex of six subunits essential in prereplication chromatin assembled at Saccharomyces cerervisiae replication origins in the G(sub.1) phase, has been studied. It is called Mcm10. Mcm10 and the MCM1-7 complex interact and thereby initiate DNA synthesis and release replication factors from origins.

DNA

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Telomeric chromatin modulates replication timing near chromosome ends

Article Abstract:

Telomeric chromatin inhibits DNA replication in a Sir3-dependent way. Saccharomyces cerevisiae telomeric DNA replicates late in the S phase. Telomeric genes are silent in transcription. Repression in Transcription of telomere-proximal genes is the result of silent chromatin initiating at the end of the chromosome. How telomeric chromatin and DNA replication relate is not known. Transcriptional repression of telomere-proximal genes is the result of silent chromatin initiating at the chromosome end, however. Mutations in a silent chromatin component, SIR3, cause telomeric DNA on chromosome V to replicate much earlier, a result of earlier initiation of Y'ARS, a close replication origin. Another telomere-proximal ARS, from an S element, does not act as an origin in a wild strain, but in a sir3 cell does so.

Telomeres, Eukaryotic cells, Cells (Biology), Eukaryotes, Chromatin, Chromosome replication

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Essential functions of amino-terminal domains in the yeast telomerase catalytic subunit revealed by selection for viable mutants

Article Abstract:

Amino terminal domains in the yeast telomerase catalytic subunit are discussed with consideration of their essential functions shown by selection for viable mutants. A large library having in it random mutations in the amino terminus of the EST2 gene, the one that encodes the Saccharomyces cerevisiae telomerase reverse transcriptases (TERTs), was set up. Certain functional alleles were selected by ability to rescue senescence of telomerase-negative cells. Analysis was carried out on 265 mutations.

Statistical Data Included, Evolution (Biology), RNA, Evolution, Telomerase, Reverse transcriptase, Mutagenesis

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