A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites
Article Abstract:
A genetic mapping system in Caenorhabditis elegans was developed based on the difference in the distribution of the transposable element Tc1 between the Bristol and the Bergerac strains. The procedure involved the selection of 40 widely distributed Tc1 sites in the Bergerac strain which are 'empty' sites in the Bristol strain. The polymerase chain reaction was used to distinguish the presence or absence of Tc1 sites, and by combining appropriate assays in a single reaction, multiple sites could be scored in a single worm. The resulting map was dense enough to allow the mapping of new mutations to chromosomal subregions from a single interstrain cross.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Mapping chromosome rearrangement breakpoints to the physical map of Caenorhabditis elegans by fluorescent in situ hybridization
Article Abstract:
A technique for mapping chromosome rearrangements to the physical map of Caenorhabditis elegans based on yeast artificial chromosomes' (YAC) hybridization patterns on meiotic nuclei is presented. YAC DNAs covering the rearrangement breakpoint were selected from the complete physical map and labeled for fluorescence in situ hybridization. Using the genetic constitution of the strain, hybridization signal patterns or number from probes in or near the genome's rearranged region can be predicted.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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The ornithine decarboxylase gene of Caenorhabditis elegans: cloning, mapping and mutagenesis
Article Abstract:
The Caenorhabditis elegans odc-1 gene and its cDNA was cloned and characterized to determine the Ornithine decarboxylase (ODC) enzyme's developmental function. An odc-1 null mutant was also isolated to offer supplementary characterization. Assay analysis of ODC activity found no substantial activity level, indicating the factor's dispensability for laboratory nematode growth.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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