A model study of factors involved in adhesion of Pseudomonas fluorescens to meat
Article Abstract:
The factors contributing to bacterial adhesion on tendon slices were determined using an in situ hybridization technique to measure adhering Pseudomonas fluorescens cells on inoculated surfaces. The results showed that adhesion did not depend on the viability of the cells. Adhesion was dependent on ionic concentration, with more cells adhering as ionic strength increased from 10 to 100 millimolar. Above this range, adhesion was sharply reduced. It is proposed that the specific sites of attachment are located within the collagen or proteoglycan molecules.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Rapid isolation and sequencing of purified plasmid DNA from Bacillus subtilis
Article Abstract:
The reduction of plasmid copy number in Bacillus subtilis during growth in the presence of chloramphenicol for plasmid selection is discussed. This involves the isolation of DNA plasmids capable of confering resistance to chloramphenicol and kanamycin from B. subtilis. Protoplast alkaline lysis and protoplast alkaline lysis magic procedures were used in the experiments. The yield of DNA plasmid was found to be related to plasmid copy number but not to chloramphenicol.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Transformation of 2,4,6-trinitrotoluene by purified xenobiotic reductase B from Pseudomonas fluorescens I-C
Article Abstract:
The enzyme xenobiotic reductase B (XenB) of Pseudomonas fluorescens I-C transforms 2,4,6-trinitrotoluene (TNT) by nitro group reduction and direct reduction of the aromatic ring. XenB belongs to the family of NAD(P)H-dependent flavoprotein oxidoreductases.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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