A most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts
Article Abstract:
Research was conducted to quantify infectious Cryptosporidium parvum oocysts using the most-probable-number (MPN) method of enumerating microorganisms. The approach involved creating a dilution series, incubating the samples for an adequate period, plating in replicate and scoring the presence of growth. Results provide evidence that the focus detection method-MPN approach is an excellent and reproducible assay for quantifying oocyst infectivity in vitro.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Use of cell culture to assess Cryptosporidium parvum survival rates in natural groundwaters and surface waters
Article Abstract:
A study is conducted to determine the survival rates of Cryptosporidium parvum in surface waters and ground-waters of Florida by using cell culture. Findings reveal that temperature, surface water or groundwater type, and the interaction of these factors has statistically significant effects on the survival of Cryptosporidium parvum.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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- Abstracts: Method for detection and enumeration of Cryptosporidium parvum oocysts in feces, manure and soils. Rapid extraction of DNA from Escherichia coli and Cryptosporidium parvum for use in PCR
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