A non-essential glutamyl aminopeptidase is required for optimal growth of Lactococcus lactis MG1363 in milk
Article Abstract:
Glutamyl aminopeptidase (PepA) produces optimal growth of the bacteria Lactococcus lactis MG1363 in milk. However, the aminopeptidase is not essential for bacterial growth. Mutants lacking PepA show normal cell density but growth during the exponential phases is slower. Escherichia coli cells expressing the pepA gene produce active PepA. The complete open reading frame (ORF) of the pepA gene encodes a protein whose N-terminal is similar to PepA isolated from the bacteria. There are no N-terminal signal sequences or hydrophobicity sites in pepA.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Molecular analysis of the regulation of nisin immunity
Article Abstract:
The putative response regulator of nisin biosynthesis, the nisR gene product, was discovered to be an essential part of the induction mechanism of the control of nisin immunity. This protein is a component of a two-part regulatory system which controls the expression of genes needed in nisin immunity and biosynthesis. Findings revealed that the 12 amino-terminal residues of the molecule are a minimum requirement for induction, with an intact ring A being a vital part.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Generation of a novel polysaccharide by inactivation of the aceP gene from the acetan biosynthetic pathway in Acetobacter xylinum
Article Abstract:
A study was conducted to analyze the identification and sequencing of aceP, a novel gene encoding a glycosyl transferase involved in acetan biosynthesis. DNA from plasmid pAG18 was prepared by centrifugation through caesium chloride/ethidium bromide gradients. A. xylinum was then grown in HS broth at 30 degrees C. In addition, the strain C1 of A. xylinum was mutagenized by exposure to methane sulfonic acid.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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