A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control
Article Abstract:
The development and optimization of a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes is reported based on the simultaneous detection of hly gene target sequences, which provides high specificity, sensitivity and quantifiability, and an internal amplification control (IAC) sequence for the assessment of PCR inhibition. This Q-PCR assay for Listeria monocytogenes is a simple and robust tool that facilitates the identification of false negatives or underestimations of contamination loads due to PCR failure.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Gateway-based destination vectors for functional analyses of bacterial ORFeomes: Application to the min system in Brucella abortus
Article Abstract:
The construction of twenty gateway-compatible destination vectors comprising of fluorescent and epitope fusion tags, various drug markers, and replication origins which could be useful for exploring existing microbial ORFeomes is reported. The vectors are observed to be useful to determine the subcellular distribution of several proteins involved in cell division and differentiation of a given bacterium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay
- Abstracts: Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin
- Abstracts: Identification of Listeria monocytogenes genes involved in salt and alkaline-pH tolerance. Involvement of the reserve material poly-beta-hydroxybutyrate in Azospirillum brasilense stress endurance and root colonization
- Abstracts: Construction of an expression system for site-directed mutagenesis of the lantibiotic mersacidin. A modified Saccharomyces cerevisiae strain that consumes L-arabinose and produces ethanol
- Abstracts: Biocontrol of food-borne pathogens Listeria monocytogenes and Salmonella enterica serovar Poona on fresh-cut apples with naturally occurring bacterial and yeast antagonists