Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay
Article Abstract:
A chemiluminescent or a colorimetric substrate for the nonisotopic detection of the ligase chain reaction (LCR) was used the polymerase chain reaction (PCR)-coupled LCR indicated a sensitivity of 10 CFU of Listeria monocytogenes. The detection technique with the chemiluminescent substrate Lumi-Phos 530 allowed detection of the LCR products in less than three hours so as to complete it within 10 hours. An there was a gradual increase in the number of LCR cycles (LCR > 10) that were examined, a ligated product was noticed with the nonisotopic detection mode when L. inocera was a target, and high background signals arose after exposure of the Lumi-Phos 530 reaction to polaroid type 612 film for 10 minutes or longer. An exposure time of 5 minutes was ideal for optimum results.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Differentiation of Listeria monocytogenes and Listeria innocua by 16S rRNA genes and intraspecies discrimination of Listeria monocytogenes strains by random amplified polymorphic DNA polymorphisms
Article Abstract:
The discrimination of Listeria monocytogenes from Listeria innocua by differences in the 16S rRna genes is discussed. The genes were amplified by polymerase chain reaction and cloned from the bacilli and their random amplified polymorphic DNA (RAPD) patterns were established. The ability of RAPD for resolving the origin of the Listeria isolates was satisfactorily demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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