A simplified subtractive hybridization protocol used to isolate DNA sequences specific to Xylella fastidiosa
Article Abstract:
A simple procedure for the subtractive hybridization isolation of DNA sequences specific to Xylella fastidiosa from diseased citrus plants was conducted. The procedure, based on the technique described by A.P. Monaco, S.A. Latt, H.D. Ochs, W. Middlesworth and M. Kunkel, resulted in the detection of the bacteria even in citrus plants that do not exhibit the disease. The isolation of Xy. fastidiosa-specific DNA from citrus shows that subtractive hybridization can be applied to other microorganisms.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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Evidence for metabolism of o-xylene by simultaneous ring and methyl group oxidation in a new soil isolate
Article Abstract:
An o-xylene-utilizing Rhodococcus, strain B3, was isolated. Results of simultaneous adaption experiments, fortuitous oxidation studies and studies of product formation by a mutant revealed that pathways for both ring and methyl group oxidation can operate in the same microorganism. It was also found that the ring oxidation pathways can be initiated by a monooxygenase-catalyzed reaction.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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The role of sulfoacetaldehyde sulfo-lyase in the mineralization of isethionate by an environmental Acinobacter isolate
Article Abstract:
The mineralization of isethionate by an environmental Acinetobacter isolate was reported. Isethionate with concentration up to 20mM was used as carbon and energy source. Sulfite-oxidizing activity were observed in isethionate-grown cells of Acinetobacter sp. ICD. Results reveal that sulfoacetaldehyde sulfo-lyase plays a significant role in the mineralization of biogenic sulfonates.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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