Additive effects of a two-amino-acid insertion and a single-amino-acid substitution in dihydropteroate synthase for the development of sulphonamide-resistant Neisseria meningitidis
Article Abstract:
Additive effects of two substitutions in dihydropteroate synthase (DHPS) for the development of sulfonamide-resistant Neisseria meningitidis are discussed. A two-amino-acid insertion and a single-amino-acid substitution, together, give new resistance, the gene for which may have been introduced by natural transformation. It was found that a normal chromosomally encoded DHPS of N. meningitidis cannot be changed to one that is sulfonamide-resistant by inserting a serine and glycine, as is seen in clinical isolates. Likely the resistance gene in the isolates evolved in another bacterial species.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
Production and pH-dependent bactericidal activity of lactocin S, a lantibiotic from Lactobacillus sake L45
Article Abstract:
Examination of the influence of various fermentation processes on the bactericidal activity of lactocin S, a lantibiotic from Lactobacillus sake L45 reveals that bacteriocin activity is maximum during the early stationary growth phase of cultures at 30 degrees Celsius. Production of bacteriocin is higher at pH5 compared with production during batch culture fermentation. Addition of 1% ethanol increases the yield of bacteriocin. The pH of the culture influences the killing of target cells mediated by lactocin S.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Transposition in Lactobacillus sakei: inactivation of a second lactocin S operon by the insertion of IS1520, a new member of the IS3 family of insertion sequences
Article Abstract:
Lactobacillus sakei is discussed relative to a transposition and inactivation of a second lactocin S operon by insertion of IS1520. IS1520 is a new member of the IS3 insertion sequences family. The new transpositionally active insertion sequence encodes two consecutive partially overlapping major ORFs. They are frameshifted in a way that is typical of the IS3 family.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Survival of Salmonellae on and in tomato plants from the time of inoculation at flowering and early stages of fruit development through fruit ripening
- Abstracts: Comparison of atomic force microscopy interaction forces between bacteria and silicon nitride substrata for three commonly used immobilization methods
- Abstracts: Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii
- Abstracts: Differential expression of three alpha-galactosidase genes and a single beta-galactosidase gene from Aspergillus niger
- Abstracts: Identificatiion and disruption of betL, a secondary glycine betaine transport system linked to the salt tolerance of Listeria monocytogenes LO28