Identification and disruption of the proBA locus in Listeria monocytogenes: role of proline biosynthesis in salt tolerance and murine infection
Article Abstract:
Intracellular accumulation of proline does not increase the infectivity of Listeria monocytogenes but does appear to increase its resistance to high salt concentrations and other environments of high osmolarity. Proline is one of the three major solutes in Listeria along with betaine and carnitine.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Mutations in the listerial proB gene leading to proline overproduction: effects on salt tolerance and murine infection
Article Abstract:
Proline does not appear to be a virulence factor or an osmolyte in Listeria monocytogenes. Mutants that overproduced proline were no more salt-tolerant or virulent in mice than the wild type.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Identificatiion and disruption of betL, a secondary glycine betaine transport system linked to the salt tolerance of Listeria monocytogenes LO28
Article Abstract:
The first genetic analysis of compatible solute transport in Listeria was conducted with the isolation, characterization and disruption of betL, a gene which plays an important role in glycine betaine uptake in L monocytogenes. The betL gene is preceded by a consensus sigma(super B)-dependent promoter and is predicted to encode a 55-kDa protein with 12 transmembrane regions. The gene also shows significant sequence homologies to other glycine betaine transporters. A betL knockout mutant which is affected in its ability to accumulate glycine betaine in the presence or absence of NaCl has been constructed in L monocytogenes and has been found to be unable to withstand concentrations of salt as high as the BetL+ parent can.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Modification of the signal sequence cleavage site of listeriolysin O does not affect protein secretion but impairs the virulence of Listeria monocytogenes
- Abstracts: Genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium Halomonas elongata DSM 3043
- Abstracts: Induction and apoptotic regression of lung adenocarcinomas by regulation of a K-Ras transgene in the presence and absence of tumor suppressor genes
- Abstracts: Detection and genotyping of Arcobacter and campylobacter isolates from retail chicken samples by use of DNA oligonucleotide arrays
- Abstracts: Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii