Adherence of Candida albicans to epithelial cells: studies using fluorescently labelled yeasts and flow cytometry
Article Abstract:
The adherence of cells of Candida albicans to epithelial cells can be determined by marking the cells with a fluorescent marker and then analyzing the epithelial cells by flow cytometry. The buccal epithelial cells whose mean fluorescence intensity per cell (MFI) is less than nonspecific fluorescence do not have any attached yeast cells while those whose MFI is higher than that of labelled yeast cells have adherent yeast cells. The yeast cells that do not adhere are removed by gating. The adherence of the cells of C. albicans to the epithelial cells is important for infection.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Hydrophobic properties of the cell surface of Candida albicans: a role in aggregation
Article Abstract:
Spectroscopic studies on three different strains of Candida (C.) albicans reveal that hydrophobicity varies in the strains and a galactose-based culture medium allows maximum hydrophobicity. The hydrophobic nature of the cell surface of C. albicans is believed to be responsible for the properties of aggregation and adhesion to biological surfaces that C. albicans exhibits, which is of importance in medicine. The techniques used involve the measurement of dielectric polarity and the amount of aggregation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Gelatin fragments block adherence of Candida albicans to extracellular matrix proteins
Article Abstract:
The non-antifungal, biocompatible peptide fragments from denatured type I collagen (gelatin) restrict the adherence of Candida albicans yeast cells to extracellular matrix glycoproteins. This helps in preventing diseases due to albicans. A 100% decrease in fungal adherence to type I collagen is obtained by the use of a gelatin peptide with 47 amino acids.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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