An alkane responsive expression system for the production of fine chemicals
Article Abstract:
Previous experiments on AlkB induction have become the bases for localizing alkS, a substance that is responsible for the encoding of the positive regulator for the alkBFGHILJKL operon on a 3.7-kb SalI-HpaI OCT plasmid fragment. Whole-cell biocatalysts, with high specific activity, resulted from the expression of the xylene oxygenase gene with the alk regulatory system usually found in recombinant strains. Prior studies were not able to locate biocatalysts that synthesize xylene monooxygenase due to the fact that these previous biocatalaysts were inadequately designed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Biocatalyst engineering by assembly of fatty acid transport and oxidation activities for in vivo application of cytochrome P-450(sub BM-3) monooxygenase
Article Abstract:
A study examined the application of whole cells containing cytochrome P-450(sub BM-3) monooxygenase for the bioconversion of long-chain saturated fatty acids to omega-1, omega-2 and omega-3 hydroxy fatty acids. Results showed that the whole-cell biotransformation of pentadecanoic acid carried out by Escherichia coli resulted in the formation of 12-, 13- and 14-hydroxypentadecanoic acids only. These products were detected primarily in the cell supernatant, indicating that the hydroxylated compounds were excreted by the cells.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Towards a biocatalyst for (S)-styrene oxide production: characterization of the styrene degradation pathway of Pseudomonas sp. strain VLB120
Article Abstract:
Research was conducted to study the styrene degradation pathway of Pseudomonas sp. strain VLB120. The DNA sequence of a genomic fragment of Pseudomonas sp. strain VLB120 was prepared from the deletion clones of pSPW1 while a Qiagen kit was utilized to determine the genomic DNA of VLB120 from styrene-grown cells. Results showed that Escherichia coli recombinants supporting styrene monooxygenase cloned genes from VLB120 transform styrene to (S)-styrene oxide.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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