At least two origins of fungicides resistance in grapevine downy mildew populations
Article Abstract:
The first case study of the evolutionary processes that lead to the emergence of quinine outside inhibiting (QoI) fungicide resistance in the plant pathogen Plasmopara viticola is presented. Sequence analysis shows that all resistant isolates carried a mutation resulting in the replacement of glycine by alanine at codon 143 (G143A).
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria
Article Abstract:
A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. The plasmid was used in Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Nitrogen-related hypermutator strain of Synechococcus sp. for use in vivo artificial evolution
Article Abstract:
Two different hypermutator strains of Synechococcus sp. strain PCC 7942 by insertionally inactivating or nutritionally repressing the DNA mismatch repair gene, mutS are constructed. Hypermutation caused by repression in PnirA-mutS transformants led to an accumulation of spectinomycin resistance mutations during growth.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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