Bioassay for nisin in milk, processed cheese, salad dressings, canned tomatoes and liquid egg products
Article Abstract:
A sensitive nisin quantification bioassay was constructed based on Lactococus lactis chromosomally encoding the nisin regulatory proteins NisK and NisR and a plasmid with a green fluorescent protein (GFP) variant gfp(Sub uv) gene under the control of the nisin-inducible nisA promoter. This strain, LAC275, was capable of transducing the signal from extracellular nisin into measurable GFP(sub uv) fluorescence through the NisRK signal transduction system.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Microplate bioassay for nisin in foods, based on nisin-induced green fluorescent protein fluorescence
Article Abstract:
This article describes construction of a plasmid containing nisin regulatory protein and a green fluorescent protein gene that can sense extracellular nisin and transduce the signal into measurable fluorescence. Results indicate that the assay can measure 2.5 and 45 nanogram and 0.9 and 1.0 microgram of nisin per milliliter of culture medium and milk, cheese and salad dressing, respectively.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
A nisin bioassay based on bioluminescence
Article Abstract:
Researches describe an assay for the antibiotic peptide nisin. The assay uses a strain of Lactococcus lactis that detects nisin and creates a bioluminescent signal. The detection limit of this assay is lower than other assays.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities
- Abstracts: Longevity regulation in Saccharomyces cerevisiae: linking metabolism, genome stability, and heterochromatin. Conjugative plasmid transfer in gram-positive bacteria
- Abstracts: Cell fate decisions within the mouse organizer are governed by graded Nodal signals. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling
- Abstracts: Use of antibiotic resistance analysis for representativeness testing of multiwatershed libraries. Mapping of genomic regions (quantitative trait loci) controlling production and quantity in industrial cultures of the edible basidiomycete Pleurotus ostereatus
- Abstracts: Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. part 2