Characterization of highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene
Article Abstract:
The cloning and characterization of four sucrose isomerase (SI) genes from isomaltulose-producing bacteria, Pantoea dispersa UQ68J, are described and the activities of the purified recombinant enzymes produced in Escherichia coli are compared. The enzyme from Pantoea dispersa is excellent when compared to other characterized SIs and it also has properties that can be used in the industrial biosynthesis of isomaltulose.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Use of pseudomonas putida EstA as an anchoring motif for display of a periplasmic enzyme on the surface of Escherichia coli
Article Abstract:
The N-terminal fusion display of the periplasmic enzyme beta-lactamase (Bla) on the surface of Escherichia coli is investigated by using translocator domain of pseudomonas putida outer membrane esterase (EstA). A topological model of the EstA translocator domain is provided, which serve as a basis for the construction of fusion proteins containing heterologous passenger domains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Cloning, nucleotide sequence and overexpression of the L-Rhamnose Isomerase gene from Pseudomonas stutzeri in Escherichia coli
Article Abstract:
Description of cloning, sequencing, overproduction and characterization of the L-Rhamnose Isomerase gene from Pseudomonas stutzeri is given. This is done to produce various kinds of rare sugars by using micro-organisms.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Characterization of thermophilic consortia from two souring oil reservoirs. Direct cloning from enrichment cultures, a reliable strategy fo isolation of complete operons and genes from microbial consortia
- Abstracts: Purification and characterization of laccase from chaetomium thermophilium and its role in humification. Growth characteristics of the thermophilic fungus Scytalidium thermophilum in relation to production of mushroom compost
- Abstracts: Characterization of Salmonella enterica serovar typhimurium from marine environments in coastal waters of Galicia (Spain)
- Abstracts: Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes. The notch target genes Hey1 and Hey2 are required for embryonic vascular development
- Abstracts: Computational definition of sequence motifs governing constitutive exon splicing. Imprinting mechanisms-it only takes two