Characterization of the chemical and antimicrobial properties of piscicolin 126, a bacteriocin produced by Carnobacterium piscicola JG126
Article Abstract:
The peptide bacteriocin, piscicolin 126, produced by the lactic acid bacterium Carnobacterium piscicola JG126, exhibits bactericidal activity against the food-borne human pathogen Listeria monocytogenes. Its molecular characterization reveals a unique amino acid sequence. Purification and structural analysis of piscicolin 126 reveals its class II bacteriocin status with 44 amino acid residues and one intrapeptide disulfide ring. The bactericidal activity of piscicolin 126 is neither affected by high pH values nor by catalase/lipase/lysozyme, but is destroyed by proteolytic enzymes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Novel method to extract large amounts of bacteriocins from lactic acid bacteria
Article Abstract:
A new method to extract large amounts of bacteriocins from lactic acid bacteria was developed. The procedure involved manipulation of the culture broth pH to maximally adsorb bacteriocins to the cell surface, which were subsequently removed by centrifugation. The procedure was used to extract pediocin AcH, nisin, sakacin A and leuconocin Lcm1 from lactic acid bacteria. The resulting bacteriocins were potent and concentrated. This demonstrates the efficacy of the new method, which will be useful in producing biopreservatives.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
User Contributions:
Comment about this article or add new information about this topic:
Novel activator of mannose-specific phosphotransferase system permease expression in Listeria innocua, identified by screening for pediocin AcH resistance
Article Abstract:
Transposon Tn917 knockout libraries are constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH in order to identify genes that are important for class IIa bacteriocin interaction and resistance in L. species. The combined results show that the inactivation of lin0142 protein is responsible for pediocin AcH resistance in G7 mutant and that lin0142 is required for the transcription of the mpt operon.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Characterization of the cellulolytic complex (cellulosome) produced by Clostridium cellulolyticum. Flux analysis of the metabolism of Clostridium cellulolyticum grown in cellulose-fed continuous culture on a chemically defined medium under ammonium-limited conditions
- Abstracts: Examination of physiological and molecular factors involved in enhanced solvent production by Clostridium beijerinckii BA101
- Abstracts: Isolation and characterization of a nitrire reductase gene and its use as a probe for denitrifying bacteria. Optimization of a reusable hollow-fiber ultrafilter for simultaneous concentration of enteric bacteria, protozoa, and viruses from water
- Abstracts: Utilization of hemin and hemoglobin by Vibrio vulnificus biotype 2. Restriction fragment length polymorphisms and sequence analysis: an approach for genotyping infectious pancreatic necrosis virus reference strains and other aquabirnaviruses isolated from Northwestern Spain
- Abstracts: Carbon monoxide, hydrogen, and formate metabolism during methanogenesis from acetate by thermophilic cultures of Methanosarcina and Methanothrix strains