Isolation and characterization of a nitrire reductase gene and its use as a probe for denitrifying bacteria

Article Abstract:

The isolation and sequencing of the heme-type nitrite reductase gene (nir) from Pseudomonas stutzeri JM300 was discussed. Analyses showed nir is the first gene in an operon followed by a gene that codes for a tetraheme protein. The strain JM300 nir also showed 88% sequence homology to P. stutzeri ZoBell nir and 67% homology to P. aeruginosa nir. A gene probe specific for denitfying bacteria was developed based on the 2.4-kb ClasI fragment within the nitrite reductase operon. Tests showed that the probe was efficient in detecting denitrifying bacteria from aquifier bioreactor samples.

Analysis, Pseudomonas, DNA probes, Microbial enzymes

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Characterization of the dominant and rare members of a young Hawaiian soil bacterial community with small-subunit ribosomal DNA amplified from DNA fractionated on the basis of its guanine and cytosine composition

Article Abstract:

Research was conducted to examine the soil bacterial community diversity of a 200-year-old site in Hawaii with expected lower diversity than communities found in continental soils due to its geographic isolation. The soil DNA was fractionated on the basis of G+C content to lower the soil bacterial diversity. Results revealed a diversity too great to be capture in a reasonable number of clones investigated which is 80 to 90 clones per soil sample.

Bacterial genetics, Ribosomal RNA, Guanine, Cytosine

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Optimization of a reusable hollow-fiber ultrafilter for simultaneous concentration of enteric bacteria, protozoa, and viruses from water

Article Abstract:

Results evaluate the usage of reusable hollow-fiber filter models to concentrate microbes from water and simultaneous recovery of other organisms from environmental samples. Data indicate recovery of multiple organisms from water with different turbidities is feasible using hollow-fiber ultrafilter.

United States, Science & research, Methods, Water, Aquatic microbiology, Environmental remediation, Ultrafiltration

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