Cloning and transcriptional analysis of the nifUHDK genes of Trichodesmium sp. IMS101 reveals stable nifD, nifDK and nifK transcripts
Article Abstract:
A study was undertaken that cloned and sequenced the nitrogenase structural nifU and nifHDK genes of the Trichodesmium sp. IMS101 for the purpose of comparing the nitrogenase proteins and nif regulatory region of Trichodesmium to other cyanobacterial diazotrophs. Nif genes are transcribed by a RNA polymerase that employs a sigma factor known as RpoN or sigma54. Results indicated that the multiple nif transcripts presented potential stem-loop structures that terminated at the transcription downstream of nifH and nifD. NifHDK transcription was unaffected by changes in light intensity.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Phylogeny of cyanobacterial nifH genes: evolutionary implications and potential applications to natural assemblages
Article Abstract:
The nifH gene encodes the two identical subunits of the Fe protein of nitrogenase. Sequences of a portion of nifH gene from diverse cyanobacteria were compared to provide additional insight into the evolution of cyanobacteria and to determine the evolutionary relationship of nitrogenase within the cyanobacteria as a group. In addition, phylogenetic analysis of nitrogenase genes provide a basis for the identification of uncultivated nitrogen-fixing cyanobacterial strains in the environment which in turn will provide molecular markers for the cultivation of desirable strains.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
New nitrogen-fixing microorganisms detected in oligotrophic oceans by amplification of nitrogenase (nifH) genes
Article Abstract:
Research was conducted to examine novel nitrogen-fixing microbial populations and diverse habitats that can support nitrogen fixation in the open ocean other than those previously reported. Nitrogenase genes were amplified from oligotrophic surface water picoplankton from the tropical Pacific and Atlantic oceans and from the Caribbean and Sargasso seas. Results demonstrate that there are diverse nitrogen-fixing phylotypes in the oceanic environment in the plankton.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Cloning and functional expression in Escherichia coli of the gene encoding the di- and tripeptide transport protein of Lactobacillus helveticus
- Abstracts: Computer-assisted laser scanning and video microscopy for analysis of Cryptosporidium parvum oocysts in soil, sediment, and feces
- Abstracts: The general secretion pathway of Erwinia carotovora subsp. carotovora: analysis of the membrane topology of OutC and OutF
- Abstracts: Thermally regulated translational control of frq mediates aspects of temperature responses in the neurospora circadian clock
- Abstracts: Estimation of methanogen biomass by quantitation of coenzyme M. A rapid and simple method for estimating sulfate reduction activity and quantifying inorganic sulfides