Computer-assisted laser scanning and video microscopy for analysis of Cryptosporidium parvum oocysts in soil, sediment, and feces
Article Abstract:
The three-dimensional spatial relationships exists between immunostained oocysts and the acridine orange-stained soil matrix material. Oocysts are suspended between soil particles, rather than being attached to them, as revealed by the hydrated, pyrophosphate-dispersed soil preparation. Distinct sporozoites are found in DIC imaging in viable oocysts with the most typical DAPI staining pattern. Computer-assisted laser scanning microscope and color video microscopy are effective techniques for examining Cryptosporidium parvum oocysts.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Assessment of a dye permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts
Article Abstract:
The capacity of dye permeability assay to identify Cryptosporidium parvum oocysts was examined by comparing the test to an in vitro excystation assay. The assays were used on sodium hypochlorite-permeabilized formaldehyde-fixed and untreated oocysts. Results show that dye permeability viability testing is not based on metabolic activity potential but on oocyst wall permeability. Results also verify the use of dye permeability assay in predicting oocyst infectivity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Influence of pretreatment and experimental conditions on electrophoretic mobility and hydrophobicity of Cryptosporidium parvum oocysts
Article Abstract:
Research was conducted to examine the effect of pretreatment and experimental conditions on the hydrophobicity and electrophoretic mobility of Cryptosporidium parvum oocysts. A further objective was to measure the effects of solution ionic strength on the hydrophobicity of polystyrene beads. Results indicate that oocysts purified using the EAPS method displayed an electrophoretic mobility that strongly depended on pH.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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- Abstracts: Effect of pasteurization on infectivity of Cryptosporidium parvum oocysts in water and milk. In vitro interactions of Asian freshwater clam (Corbicula fluminea) hemocytes and Cryptosporidium parvum oocysts
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