Cloning, expression, and characterization of the katG gene, encoding catalase-peroxidase, from the polycyclic aromatic hydrocarbon-degrading bacterium Mycobacterium sp. strain PYR-1
Article Abstract:
Research describes the construction of an oligonucleotide probe that contains part of the gene encoding catalase-peroxidase to screen the genomic library of Mycobacterium sp. strain PYR-1. Data show that the enzyme is involved in the polycylic aromatic hydrocarbon metabolism of the bacterium as indicated by its induction in pyrene-containing cultures.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Purification of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Pseudomonas putida OU83 and characterization of the gene (bphC)
Article Abstract:
The bphC gene from Pseudomonas putida OU83's PCB pathway is cloned and sequenced to apparent homogeneity. The gene product is then purified and compared with the 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-DBPD) proteins from the rest of the bphC gene family. Results show that 2,3-DBPD is an octamer of identical subunits. The data also suggest that its protein sequence consists of 292 amino acid residues with a calculated molecular mass of 31.9 kDa.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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- Abstracts: Molecular cloning, nucleotide sequence, and expression of genes encoding a polycyclic aromatic ring dioxygenase from Mycobacterium sp. strain PYR-1
- Abstracts: Inactivation of an ABC transporter gene, mcyH, results in loss of microcystin production in the cyanobacterium microcystis aeruginosa PCC 7806
- Abstracts: Sequence analysis of the Lactococcus lactis temperate bacteriophage BK5-T and demonstration that the phage DNA has cohesive ends