Cloning of Phanerochaete chrysosporium leu2 by complementation of bacterial auxotrophs and transformation of fungal auxotrophs
Article Abstract:
The expression vector lambdaYES was used to construct a copyDNA library for Phanerochaete chrysosporium . This vector has a lacZ promoter and GAL1 promoter for the expression of both Escherichia coli and Saccharomyces cerevisiae, respectively. These characteristics enabled the complementation of auxotrophs for E. coli and yeast and allowed for the cloning of the leu2 gene as well as other cDNAs involved in biosynthetic pathways. A potential application for this would be in the transformation of P. chrysosporium and other wood-degrading fungi.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Development and characterization of a xylose-dependent system for expression of cloned genes in Bacillus subtilis: conditional complementation of a teichoic acid mutant
Article Abstract:
A new robust expression system for Bacillus subtilis conditional complementation is presented, contained on plasmid pSWEET, which is a derivative of pDG364 (3). Testing involved comparing the system to the isopropyl-beta-D-thiogalactopyranoside-induced spac system, which determined that the xylose-dependent expression derived from pSWEET had a greater modulated expression capacity, a higher induction/repression ratio, and lower expression levels if no inducer is present.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis
Article Abstract:
Research was conducted to study several food-grade bacterial strains of the genus Bacillus subtilis for extracellular phytase production. Strains were evaluated for phytase production in Luria broth while bacteria prepared on wheat bran extract were gathered by centrifugation. Moreover, the reaction pH was represented as a function of relative activity. Results showed that all cloned and sequenced microbial phytases were homologous to each other.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Unwinding dosage compensation. Trans-sensing effects: the ups and downs of being together. Bugs on drugs go GAGAAA
- Abstracts: Growth competition between Halobacterium salinarium strain PHH1 and mutants affected in gas vesicle synthesis
- Abstracts: Evolution of the chromosomal location of rDNA genes in two Drosophila species subgroups: Ananassae and melanogaster
- Abstracts: A new broad-spectrum protease inhibitor from the entomopathogenic bacterium Photorhabdus luminescens. Occurrence of natural dixenic associations between the symbiont Photorhabdus luminescens and bacteria related to Ochrobactrum spp. in tropical entomopathogenic Heterorhabditis spp. (Nematoda, Rhabditida)
- Abstracts: Characterization of thermophilic consortia from two souring oil reservoirs. Direct cloning from enrichment cultures, a reliable strategy fo isolation of complete operons and genes from microbial consortia