Cloning of the genes for degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine from Rhodococcus sp. strain TE1
Article Abstract:
The plasmid pKL1 present in the Rhodococcus strain TE1 contains two genes eptA and atrA which enable it to degrade the herbicides S-ethyl dipropylthiocarbamate (EPTC) and atrazine respectively. The strain TE3 is incapable of degrading these herbicides but when pKL1 was cloned and transferred into it, TE3 acquired the capacity to degrade EPTC and atrazine. However pKL1 does not have any affect in Escherichia coli. The genes eptA and atrA are structural genes and are responsible for producing the enzymes required for the degradation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Characterization of the expression of the thcB gene, coding for a pesticide-degrading cytochrome P-450 in Rhodococcus strains
Article Abstract:
The thcR gene codes for a positive regulatory protein are necessary for the expression of the thcB gene in Rhodococcus. The tchB gene expression increases in the presence of the thiocarbamate pesticides such as s-ethyl dipropylthiocarbamate and glucose. The gene expression decreases in the presence of cysteine but is unaffected by atrazine, simazine and carbofuran. The thcB, thcC and thcD genes encode the cytochrome P-450 system, involved in the degradation of thiocarbamates and other pesticides in Rhodococcus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Metabolism of the herbicide atrazine by Rhodococcus strains
Article Abstract:
The degradation of the herbicide atrazine is affected by Rhodococcus strains. Atrazine is metabolized only by rhodococci that degrade the herbicide EPTC (s-ethyl-dipropylthiocarbamate). Deethyl- and deilsopropylatrazine are produced when Rhodococcus strain TE1 metabolilzes atrazine under aerobic conditions. The other s-triazine herbicides, propazine, simazine and cyanazine, are also metabolized by the bacterium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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