Construction and characterization of a 1,3-propanediol operon
Article Abstract:
A study was conducted to determine the characterization of the genes and enzymes responsible for the conversion of glycerol to 1,3-propanediol (PD) operon. Enhancement of the utility of the 1,3-PD genes was analyzed through their rearrangement into a form that would be adaptable to expression in different hosts under any desired regulation. The study revealed that the 1,3-PD operon was designed so that it can be readily altered for expression in other prokaryotic hosts and is therefore helpful in metabolic engineering of 1,3-PD pathways from glycerol and other substrates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Use of an ipb-lux fusion to study regulation of the isopropylbenzene catabolism operon of Pseudomonas putida RE204 and to detect hydrophobic pollutants in the environment
Article Abstract:
Plasmid pOS25 was constructed to investigate the regulation of the isopropylbenzene (ipb) catabolism operon. The plasmid contains the regulatory elements of the ipb operon, namely ipbR and ipbo/p. Inducers of the ipb operon were found to be hydrophobic compounds and were also observed to include monoalkylbenzenes, substituted benzenes and toluenes, alkanes and cycloalkanes, chlorinated solvents and naphthalenes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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