Construction and complementation of In-frame deletions of the essential Escherichia coli thymidylate kinase gene
Article Abstract:
A study in which the tmk gene was replaced by kanamycin resistance gene kka1, which encodes the amino glycoside 3'-phosphotransferase kanamycin kynase is presented. Growth of transgenic Escherichia coli strains is dependent on thymidylate kinase activities of various origin expressed from plasmids and the construction of Escherichia coli in-frame deletion strains of tmk, which encodes thymidylate kinase, Tmk is reported.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
A single-nucleotide-polymorphism-based multilocus genotyping assay for subtyping lineage I isolates of Listeria monocytogenes
Article Abstract:
The isolates of Listeria monocytogenes, an intracellular pathogen of food-borne disease, were subjected to multilocus genotyping assay (MLGT) to achieve a rapid identification of the subtypes in order to develop effective control measures for this pathogen. The method proved to be a powerful tool to identify the pathogens and for other epidemiological investigations.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Recombination and clonal propagation in different populations of the lichen Lobaria pulmonaria. Testing for clonal propagation
- Abstracts: Imprinting and the initiation of gene silencing in the germ line. Nuclear reprogramming by human embryonic stem cells
- Abstracts: Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution. Detection of genetic markers of fecal indicator bacteria in Lake Michigan and determination of their relationship to Escherichia coli densities using standard microbiological methods
- Abstracts: Cloning and characterization of the polyhydroxybutyrate depolymerase gene of Pseudomonas stutzeri and analysis of the function of substrate-binding domains
- Abstracts: Cloning, characterization, and molecular application of a beta-agarase gene from vibrio sp. strain V134. Statistical analyses: Possible reasons for unreliability of source tracking efforts