Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution
Article Abstract:
Quantitative PCR assays are developed for the detection of bovine feces-specific genetic markers and a method is designed for the enumeration of these markers by using a Markov chain Monte Carlo approach. Acceptable assay performance combined with the prevalence of DNA targets across different cattle populations have shown that these quantitative assays are useful in monitoring bovine fecal pollution in ambient waters.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
User Contributions:
Comment about this article or add new information about this topic:
Single-nucleotide polymorphism phylotyping of Escherichia coli
Article Abstract:
A rapid and easily automated phylogenetic grouping technique based on analysis of bacterial genome single-nucleotide polymorphisms (SNPs) was developed. The results indicated that the information for the 13 SNPs are sufficient to construct the phylogenic history of Escherichia coli and that the SNP phylotyping approach are suitable and robust for phylogenic analyses of a wide variety of bacterial species.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
Detection of genetic markers of fecal indicator bacteria in Lake Michigan and determination of their relationship to Escherichia coli densities using standard microbiological methods
Article Abstract:
Lake Michigan surface waters impacted by fecal pollution are assessed to determine the occurrence of genetic markers for Bacteroides and Escherichia coli. This analysis shows the usefulness of non-culture-based microbial-source tracking approaches and the prevalence of these genetic markers in the Great Lakes, including freshwater coastal beaches.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: C.elegans HIM-17 links chromatin modification and competence for initiation of meiotic recombination. Around the world of DNA damage INO80 days
- Abstracts: Testing for segregation distortion in genetic scoring data from backcross or doubled haploid populations. Nuclear mitochondrial pseudogenes as molecular outgroups for phylogenetically isolated taxa: A case study in Sphenodon
- Abstracts: Mechanism of force generation of a viral DNA packaging motor
- Abstracts: Imprinting and the initiation of gene silencing in the germ line. Nuclear reprogramming by human embryonic stem cells
- Abstracts: Specific oligonucleotide primers for detection of lecithinase-positive Bacillus spp. by PCR. Rapid and quantitative detection of hepatitis A virus from green onion and strawberry rinses by use of real-time reverse transcription-PCR