alpha- and beta-proteobacteria control the consumption and release of amino acids on lake snow aggregates

Article Abstract:

alpha- and beta-Proteobacteria seem to predominate in lake snow aggregates, where they consume dissolved amino acids in the early phase of the aggregate but release amino acids during the late phase. Lake snow consists of macroscopic aggregates of living and dead algae, zooplankton molts and other organic and inorganic debris.

Microbial metabolism, Microbial mats

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Identification in situ and dynamics of bacteria on limnetic organic aggregates (lake snow)

Article Abstract:

Members of the domain Bacteria constitute 60% or more of the microbial cells colonizing limnetic macroscopic organic aggregates. The aggregates were collected in situ or formed of natural lake water in rolling cylinders under simulated in situ conditions and analyzed using rRNA-directed fluorescent oligonucleotide probes. Staining studies with 4',6-diamidino-2-phenylindole indicated that the beta-subclass proteobacteria were more abundant than the alpha and gamma-subclasses. The beta-subclass proteobacteria increased with time during time course experiments with aggregates made in the laboratory.

Analysis, Usage, Nucleic acid probes, Microbial populations, Lake ecology

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Phylogenetic analysis and in situ identification of bacteria in activated sludge

Article Abstract:

The phylogenetic analysis and in situ identification of bacteria in activated sludge was examined using of the ribosomal RNA (rRNA) approach. Full-length genes coding for the small-subunit rRNA were amplified by the polymerase chain reaction and cloned to the pGEM-T vector. Results show that activated sludge sample contains cells from another unsequenced genotype that has just one of two signatures for the genus Arobacter.

Bacteria, Polymerase chain reaction, Ribosomal RNA, Phylogeny, Sludge

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