DNA extraction and PCR methods for the detection of Listeria monocytogenes in cold-smoked salmon
Article Abstract:
Four rapid detection protocols for detecting Listeria monocytogenes in cold-smoked salmon have been developed. Hexadecyl trimethylammonium bromide (CTAB) extraction, CTAB extraction with ether separation, CTAB extraction with column purification and CTAB extraction with particle separation and column purification can detect Listeria monocytogenes in less than 36 h via DNA extraction followed by polymerase chain reaction. They are faster than conventional detection methods and have the potential for automation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Development of a PCR-enzyme immunoassay oligoprobe deection method for Toxoplasma gondii oocysts, incorporating PCR controls
Article Abstract:
Research describes the development of a polymerase chain reaction (PCR) amplification and detection method for Toxoplasma gondii oocyst nucleic acids. The method uses uracil-N-glycosylase to prevent false-positives, an internal control to identify false-negatives, and an oligoprobe for the detection of nucleic acids using the PCR-enzyme immunoassay.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms
Article Abstract:
The use of peptide nucleic acid (PNA) probes as a superior alternative to DNA probes, especially when used for environmental samples is described. The PNA probes performed better than the DNA probes in all experiments, in terms of both target specificity and signal intensity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Use of molecular typing methods to trace the dissemination of Listeria monocytogenes in a shrimp processing plant
- Abstracts: Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization. L. monocytogenes oligonucleotide probe
- Abstracts: Invasions of P elements. An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster
- Abstracts: Different strategies for molecular differentiation of Mycpbacterium bovis strains isolated in Sardinia, Italy
- Abstracts: Distinct replication requirements for the two Vibrio cholerae chromosomes. The making and breaking of sister chromatid cohesion