Design and application of highly responsive fluorescence resonance energy transfer biosensors for detection of sugar in living Saccharomyces cerevisiae cells
Article Abstract:
Combinatorial engineering of the domain linker and the binding protein moiety are used for developing a protein sensor with a highly responsive fluorescence resonance energy transfer (FRET) signal for sensing sugars in living Saccharomyces cerevisiae cells. Highly responsive FRET images are generated from real-time analysis of maltose uptake of Saccharomyces cerevisiae when optimized maltose sensors are used as in vivo sensors.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Secretion of recombinant pediocin PA-1 by Bifidobacterium longum, using the signal sequence for Bifidobacterial alpha-amylase
Article Abstract:
A recombinant DNA, encoding the chimeric protein of the signal sequence for bifidobacterial alpha-amylase mature pediocin PA-1, was introduced into Bifidobacterium longum MG1. Biologically active pediocin PA-1 was successfully secreted from the strain and showed bactericidal activity against Listeria monocytogenes and the same molecular mass as native pediocin PA-1.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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