Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA
Article Abstract:
Former primer 63f and reverse primer 1387r were designed to amplify approximately 1,300 bp of a consensus 16S rRNA gene from bacteria. The efficacy and specificity of these two PCR primers were tested by using them to amplify 16 rRNA genes from template DNA taken from a wide range of organisms, including Eubacterium species and organisms from the coryneform and Micrococcus genera. The amplimer pair 63f-1387r was found to be more successful than the amplimer pair 27f-1392r in amplifying the 16S rRNA genes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Molecular evidence for the evolution of metal homeostasis genes by lateral gene transfer in bacteria from the deep terrestrial subsurface
Article Abstract:
Lateral gene transfer (LGT) plays a vital role in increasing the genetic diversity of microorganisms and promoting the spread of fitness-enhancing phenotypes throughout microbial communities. A study is presented in which phylogenetic analyses is used to examine the role of LGT in the evolution of metal homeostasis in subsurface bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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