Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization
Article Abstract:
The utilization of reverse transcription and polymerase chain reaction for the detection in activated sludge and surface waters of enteroviruses is discussed. RT-PCR was compared against direct hybridization and cell culture methods. The sensitivity of RT-PCR was overwhelmingly better compared to the other two. However, contaminants in activated sludge and concentrated surface waters must be monitored to avoid the inhibition of its enzymatic amplification.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR
Article Abstract:
Research was conducted to illustrate the synthesis of multiple extracellular lipases in Candida rugosa. To quantify the expression of lip genes, a competitive reverse transcription-PCR assay was developed. Using the approach made it possible to demonstrate the differential expression of the five lip genes in the presence of different inducers that are known to be able to increase C rugosa lipase production.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR
Article Abstract:
Reverse transcriptase PCR was used to identify acute bee paralysis virus (ABPV) and black queen cell virus (BQCV) with sensitivities of 1,600 genome equivalents of ABPV and 130 genome equivalents of BQCV. This method is more rapid than serological methods.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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- Abstracts: Evaluation of removal of noroviruses during wastewater treatment, using real-time reverse transcription-PCR: different behaviors of genogroups I and II
- Abstracts: Identification of an asymmetrically localized determinant, Ash1p, required for lineage-specific transcription of the yeast HO gene
- Abstracts: Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Detection of DNA damage in prokaryotes by terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling
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- Abstracts: Rapid and sensitive method for detection of shiga-like toxin-producing Escherichia coli in ground beef using the polymerase reaction