Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells
Article Abstract:
Research was conducted to examine the relationship between the cellular viability and detection of mRNA in Escherichia coli in cells killed by ethanol or heat. For the detection of mRNA from rpoH, groEL and tufA genes, reverse transcription-PCR (RT-PCR) methods were developed. mRNA targets were detected immediately in cells killed by ethanol or heat but disappeared at room temperature. Results are in good agreement with the hypothesis that mRNA is the most promising indicator of viability in bacteria. However, its persistence in dead cells varies according to certain conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Role of the DmpR-mediated regulatory circuit in bacterial biodegradation properties in methylphenol-amended soils
Article Abstract:
Research indicates that the ability of DmpR-like regulators to control aromatic catabolic pathways may be due to the ability to quickly adapt regulator properties to various polluting compound profiles. Studies were conducted on two different soil types using the wild-type system as well as an isogenic counterpart with an enhanced sensitivity to para-substituted phenols.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Detection of DNA damage in prokaryotes by terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling
Article Abstract:
Researchers describe a technique to detect 3'-OH DNA ends, which are created during DNA repair. The technique involves enzymatically labeling these ends with dUTP-fluorescein isothiocyanate. Fluorescence microscopy can detect the number of ends, which would be an indicator of DNA damage in prokaryotes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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- Abstracts: Comparison of vero cell assay and PCR as indicators of the presence of verocytotoxigenic Escherichia coli in bovine and human fecal samples
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