Development of PCR primers from internal transcribed spacer region 2 for detection of Phytophthora species infecting potatoes
Article Abstract:
PCR primers and assay techniques have been established to detect and identify three Phytophthora species which infect potatoes and cause late blight and pink rot diseases. Primers based on sequence analysis of internal transcribed spacer region 2 of ribosomal DNA resulted into PCR products of 456 bp (P. infestans), 136 bp (P. erythroseptica) and 455 bp (P. nicotianae) and were employed to detect the pathogens in potato leaf (P. infestans) and tuber (P. infestans, P. erythroseptica, and P. nicotianae) tissue with a sensitivity limit of 1 to 10 pg of DNA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Use of selective capture of transcribed sequences to identify genes preferentially expressed by Streptococcus suis upon interaction with porcine brain microvascular endothelial cells
Article Abstract:
The selective capture of transcribed sequences (SCOTS) is used to identify 28 genes preferentially expressed by the major swine pathogen and zoonotic agent Streptococcus suis upon interaction with porcine brain microvascular endothelial cells. It is demonstrated that SCOTS is suitable for the elucidation of gene expression in streptococcal species and can contribute to a better understanding of the pathogenesis of S. suis infections.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
A cyclic AMP receptor protein-regulated cell-cell communication system mediates expression of a FecA homologue in Stenotrophomonas maltophilia
Article Abstract:
Biological processes or other targeted genes controlled by the rpf/DSF system in Stenotrophomonas maltophilia were investigated. The rpf/DSF system regulated the expression of a FecA homologue, the rpf/DSF system was shown to be regulated by RpfC through protein-protein interaction and crp encoding cyclic AMP (cAMP) receptor protein (CRP) resulted in reduced FecA expression rpfF transcript level.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Development of a universal diluting fluid for membrane filtration sterility testing. A rapid, specific membrane filtration procedure for enumeration of enterococci in recreational water
- Abstracts: Molecular comparisons of freshwater and marine isolates of the same morphospecies of heterotrophic flagellates
- Abstracts: Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples
- Abstracts: Pleitropic role of quorum-sensing anti-inducer 2 in Photorhabdus luminescenes. Nickel-resistant determination from Leptospirollim ferriphilum
- Abstracts: Reconstitution of an SOS response pathway: derepression of transcription in response to DNA breaks. A new function for p53 ubiquitination