Reconstitution of an SOS response pathway: derepression of transcription in response to DNA breaks
Article Abstract:
Double-stranded DNA (dsDNA) breakage in Escherichia coli typically occurs during DNA replication and triggers transcription of around 20 DNA repair and recombination genes in what is called the SOS response. In response to dsDNA breaks, purified RecA protein, RecBCD enzyme, single-stranded DNA-binding protein and LexA repressor derepresses transcription from an SOS promoter. Derepression is quicker if the DNA with the dsDNA damage comes with a chi recombination hot spot. This implies a unique regulatory role for one of the most common octaimers in the Escherichia coli genome.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1998
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A new function for p53 ubiquitination
Article Abstract:
The ubiquitination under certain conditions have contributed to transcriptional activation rather than protein stability of the tumor-suppressor protein p53, a key regulator of cell-cycle control, apoptosis and genomic stability. The ubiquitination of p53 is found to occur in response to stimuli such as DNA damage that results in stabilization of p53 and it is promoted by the transcription factor E4F1, a zinc-finger protein of the GL1/Kruppel family.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2006
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Active transcriptional repression by the Rb-E2F complex mediates G1 arrest triggered by p16(super INK4a), TGF-beta, and contact inhibition
Article Abstract:
A study was conducted to show that retinoblastoma protein needs to interact with the E2F family of transcription factors to arrest cells in the G1 phase. Cells were transfected utilizing the calcium phosphate technique. The cells were then harvested and fixed in ethanol before being treated with propidium iodide. It was also shown that the arrest correlates with the active transcriptional repression by the protein.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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