Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method
Article Abstract:
The study aims to develop and optimize a protocol for the rapid detections of Escherichia coli O157:H7 in aqueous samples by a combined immunomagnetic bead-immunoliposome (IMB/IL) fluorescence assay. The use of this procedure is expected to increase the speed by which E. coli O157:H7 might be identified in aqueous specimens linked to epidemiological investigation and might thus increase the speed by which health care facilities and the population at large might be alerted of possible health threats.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Microbiological transformations of lipids: acyl-specific hydrolysis of lard by yeasts
Article Abstract:
The result of lard hydrolysis by cultures of Saccharomyces cerevisiae and Schizosaccharomyces octosporus is discussed. Gas-liquid chromatography was sued to determine fatty acid production. The positional specifities of the lipases of both organisms were determined by synthetic triglycerides. Results indicate that valuable and usable pure fatty acids can be biotranformed to produce yeast hydrolytic enzymes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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A model to esimate the optimal sample size for microbiological surveys
Article Abstract:
A statistical model based on a Bayesian mode of inference for the estimation of the sample size for an accurate analysis of bacterial subtypes in a specimen. The model gives probability distribution of strains in a specimen that is used to estimate the probability of identifying all strains present in the sampled colonies.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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- Abstracts: Development of a sensitive chemiluminometric assay for the detection of beta-galactosidase in permeabilized coliform bacteria and comparison with fluorometry and colorimetry
- Abstracts: Colorimetric immuno-protein phosphatase inhibition assay for specific detection of microcystins and nodularins of cyanobacteria
- Abstracts: Measurement of antimicrobial-resistant Escherichia coli in pig feces with a hydrophobic grid membrane filter interpreter system
- Abstracts: Development of a self-cloning system for Actinomadura verrucosospora and identification of polyketide synthase genes essential for production of the angucyclic antibiotic pradimicin
- Abstracts: Activity of the plasma membrane H+ -ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid