Different forms of TFIIH for transcription and DNA repair: holo-TFIIH and a nucleotide excision repairosome
Article Abstract:
Experimental studies of the structure-function relationship of the RNA polymerase transcription factor IIH (TFIIH) in yeast reveal that TFIIH exists in two distinct forms for nucleotide excision repair (NER) and for transcription. TFIIH involved in transcription comprises of three components, the SSL2 gene product, a 5-subunit core and a protein complex of 45, 47 and 33 kDa polypeptides that displays protein kinase activity. The TFIIH for NER does not contain the kinase complex but has RAD4, RAD14, RAD1, RAD1O and RAD2 gene products and is more potent than the polypeptides during NER.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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Electron crystal structure of an RNA polymerase II transcription elongation complex
Article Abstract:
An RNA polymerase II transcription elongation complex electron crystal structure, as determined by electron crystallography, is discussed. The DNA seems to have roughly 90-degree bend between downstream and upstream regions and lies entirely on one face of the polymerase, it seems. It had been thought to go through a hole to the other side. The structure was determined when the complex was actively transcribing and had in it the yeast RNA polymerase II associated with template DNA and product RNA.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1999
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Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH
Article Abstract:
Research describes two-dimensional crystallographic analysis of the "core" TFIIH transcription factor complex. Data reveal that TFIIH core consists of 5 subunits that are required for both transcription and DNA repair.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 2000
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