Direct detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment
Article Abstract:
A simple yet fast procedure by means of the reverse transcriptase-polymerase chain reaction (RT-PCR) approach was developed to detect viable bacteria, molds, and yeasts in milk samples after treatment. The RT-PCR method proved to be a potent tool in detecting viable contaminants because of its high potential to increase detection sensitivities and speed, with the detection level placed at 10 cells ml of milk(super -1). Because of its rapidity and simplicity, it can be easily transposed to other foods and other contaminants.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Identification and detection of Bacillus sporothermodurans spores in 1, 10, and 100 milliliters of raw milk by PCR
Article Abstract:
A new method of detecting Bacillus sporothermodurans in raw milk using polymerase chain reaction is presented. B. sporothermodurans is a gram-positive bacterium with endospores that are resistant to sterilization and ultrahigh temperature treatment of raw milk. The method relies on subtractive hybridization to obtain specific sequences of the bacterium's DNA. The detection limits are 9, 0.4 and 0.22 CFU/ml. for 1, 10, and 100 ml. of raw milk.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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