Effect of L-proline on sake brewing and ethanol stress in Saccharomyces cerevisiae
Article Abstract:
The role of intracellular L-proline in cells of Saccharomyces cerevisiae grown under ethanol stress is evaluated. The strain that could accumulate L-proline was used to brew sake containing five times more L-proline than what is found in sake crewed with the control strain, without affecting the fermentation profiles.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Gene dosage effect of L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in Saccharomyces cerevisiae
Article Abstract:
The gene dosage effect of three L-proline biosynthetic enzymes on L-proline accumulation and freeze tolerance in a non-L-proline-utilizing strain is examined. Overexpression of the wild-type enzymes has no influence on L-proline accumulation, which suggests that the complex is very unstable in nature.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Desensitization of feedback inhibition of the Saccharomyces cervisiae [gamma]-glutamyl kinase enhances proline accumulation and freezing tolerance
Article Abstract:
Enzymatic analysis of recombinant proteins was carried out and Saccharomyces cervisiae was subjected to feedback inhibition by proline. It was seen that yeast cell expressing mutant [gamma]-glutamyl kinase were more tolerant to freezing stress than cells expressing the Asp154Asn mutant.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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